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. 2015 Jul 31;10(7):e0133739. doi: 10.1371/journal.pone.0133739

Fig 6. Optimized P145-ST reaction for increase of S2 glycan generation.

Fig 6

For increase of S2 glycan content (%), additional CMP-NeuAc (A) or AP (B) was added 30 min after the beginning of the P145-ST reaction. Further increase of S2 glycan content was achieved by the simultaneous addition of CMP-NeuAc and AP at 30 min of the reaction (C). Through two sequential additions of both CMP-NeuAc and AP at 30 and 120 min of the reaction, relative content of S2 glycan increased up to 98% (D). Arrows indicate the time points for additions of CMP-NeuAc and/or AP. Open circles and filled squares represent the relative contents (%) of S2 glycan generated by the reactions with and without the supplemental addition respectively.