Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Jul 31;10(7):e0134523. doi: 10.1371/journal.pone.0134523

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 Fan et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

Fig 2 — After being stained with different fluorescent antibodies, the CD3+CD4+CXCR5+CD45RA- T cells were gated. Subsequently, memory Tfh cells were gated on CCR7 expression and the frequency of CCR7+, CCR7+ICOS+, CCR7- and CCR7-ICOS+ memory Tfh cells was analyzed by flow cytometry and the numbers of each type of cells were calculated. (A) Flow cytometry analysis. (B) The numbers of CCR7+ memory Tfh cells in the HC, MS patients pre- and post-treatment. (C) The numbers of CCR7+ memory Tfh cells in the MS-CR patients pre- and post- treatment. (D) The numbers of CCR7+ memory Tfh cells in the MS-PR patients pre- and post-treatment. (E) The numbers of CCR7+ICOS+ memory Tfh cells in the HC, MS patients pre- and post-treatment. (F) The numbers of CCR7+ICOS+ memory Tfh cells in the MS-CR patients pre- and post-treatment. (G) The numbers of CCR7+ICOS+ memory Tfh cells in the MS-PR patients pre- and post-treatment. (H) The numbers of CCR7- memory Tfh cells in the HC, MS patients pre- and post-treatment. (I) The numbers of CCR7- memory Tfh cells in the MS-CR patients pre- and post- treatment. (J) The numbers of CCR7- memory Tfh cells in the MS-PR patients pre- and post-treatment. (K)The numbers of CCR7-ICOS+ memory Tfh cells in the HC, MS patients pre- and post-treatment. (L) The numbers of CCR7-ICOS+ memory Tfh cells in the MS-CR patients pre- and post-treatment. (M) The numbers of CCR7-ICOS+ memory Tfh cells in the MS-PR patients pre- and post-treatment. There was no significant difference in the numbers of CCR7-PD-1+, CCR7-PD-1+ICOS+, CCR7-CD40L+, CCR7+PD-1+, CCR7+PD-1+ICOS+, CCR7+CD40L+ memory Tfh cells between the patients and HC as well as in MS patients before and after treatment (data not shown). The horizontal lines indicate the median values for each group.