Table 2. Fitted Two-Site Exchange Parameters.
kex (s–1) | pb (%) | ka (s–1) | kb (s–1) | |
---|---|---|---|---|
WT E:THF:NADPH | 1890 ± 80 | 0.69 ± 0.02 | 1880 ± 80 | 13.0 ± 0.3 |
WT E:THF:NADP+ | 1420 ± 70 | 1.25 ± 0.07 | 1400 ± 70 | 17.7 ± 0.4 |
L28F E:THF:NADPH | 770 ± 20 | 2.75 ± 0.04 | 740 ± 20 | 21.0 ± 0.3 |
L28F E:THF:NADP+ | 910 ± 20 | 2.67 ± 0.04 | 890 ± 20 | 24.3 ± 0.3 |