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. 2015 Jul 6;137(29):9459–9468. doi: 10.1021/jacs.5b05707

Table 2. Fitted Two-Site Exchange Parameters.

  kex (s–1) pb (%) ka (s–1) kb (s–1)
WT E:THF:NADPH 1890 ± 80 0.69 ± 0.02 1880 ± 80 13.0 ± 0.3
WT E:THF:NADP+ 1420 ± 70 1.25 ± 0.07 1400 ± 70 17.7 ± 0.4
L28F E:THF:NADPH 770 ± 20 2.75 ± 0.04 740 ± 20 21.0 ± 0.3
L28F E:THF:NADP+ 910 ± 20 2.67 ± 0.04 890 ± 20 24.3 ± 0.3