Table 1. Bacterial strains and plasmids used in this study.
| Strain or Plasmid | Relevant characteristics | Source or Reference |
|---|---|---|
| P. aeruginosa | ||
| PAO1 | Wild type | ATCC15692 |
| fprB | fprB mutant, derivative of PAO1 in which fprB was disrupted by pKNOCKfprB, Gmr | This study |
| Fur | fur mutant | [58] |
| ΔiscR | iscR mutant, derivative of PAO1 in which a part of iscR was deleted | [32] |
| finR | finR mutant, derivative of PAO1 in which finR was disrupted by pKNOCKfinR, Gmr | This study |
| oxyR | oxyR mutant, derivative of PAO1 in which oxyR was disrupted by pKNOCKoxyR, Gmr | This study |
| soxR | soxR mutant, derivative of PAO1 in which soxR was disrupted by pKNOCKsoxR, Gmr | [33] |
| E. coli | ||
| DH5α | ϕ80d lacZΔM15, recA1, endA1, gyrA96, thi-1, hsdR17(rk −, mk +), supE44, relA1, deoR, Δ(lacYMA-argF)U169 | Stratagene Inc. (USA) |
| BW20767 | leu-63::IS10 recA1 creC510 hsdR17 endA1 zbf-5 uidA(ΔMlu1)::pir+ thi RP4-2-tet::Mu-1kan::Tn7 | [59] |
| Plasmids | ||
| pKNOCKGm | Suicide vector, Gmr | [53] |
| pKNOCKfprB | pKNOCKGM containing a 247-bp fragment of fprB encoding region, Gmr | This study |
| pKNOCKfinR | pKNOCKGM containing a 284-bp fragment of finR encoding region, Gmr | This study |
| pKNOCKoxyR | pKNOCKGM containing a 291-bp fragment of oxyR encoding region, Gmr | This study |
| pBBR1-MCS4 | Medium-copy-number expression vector, Apr | [55] |
| pFprB | pBBR1MCS-4 containing fprB, Apr | This study |
| pISC | pBBR1MCS-4 containing iscSUA-hscBA-fdx2-iscX coding regions, Apr | [32] |
| pIscR | pBBR1MCS-4 containing iscR, Apr | [32] |
| pUC18-miniTN7T::GM-lacZ | Transcriptional fusion vector with Tn7 insertion at a single site on the chromosome | [60] |
| pPfprB | pUC18-miniTN7T::GM-lacZ with fprB-promoter insertion at MCS | This study |
| pPfprBSD | pUC18-miniTN7T::GM-lacZ with site-directed mutagenic fprB-promoter insertion at MCS | This study |
Gmr, gentamicin resistance; Apr, ampicillin resistance