Fig 7. Effect of mitofusin deficiency on respiratory complexes protein level in MEF cells.
The cells were grown in the medium supplemented with FBS. Selected respiratory complexes proteins were detected in MEFwt and MEFMfn2-/- cells by Western blotting. Representative blot of selected mitochondrial complexes subunits and densitometric analysis of at least six independent experiments are shown. Data are presented as mean immunoreactivity normalized to immunoreactivity of PCNA ± S.D. Immunoreactivity of these proteins in wild type cells is assumed as 100%, n = 6–8, *p<0.05.