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. 2015 Jul 31;9(7):e0003963. doi: 10.1371/journal.pntd.0003963

Fig 7. Examination of aliquots of urinary sediment (pellets) from S. haematobium-positive patients´ urine samples by LAMP.

Fig 7

Figure shows the LAMP results (up, by color change; down, by agarose electrophoresis) when using aliquots of 100 μL of pellets to obtain DNA as template by using (A) the i-genomic Urine DNA Extraction Mini Kit (Intron Biotechnology, UK); (B) the heating NaOH-SDS method and (C) the rapid heating method-the rapid-heat LAMPellet method-. Lanes M: 50 bp DNA ladder (Molecular weight marker XIII, Roche); lanes Sh: genomic DNA from S. haematobium (1 ng); lanes 1–18: S. haematobium-positive samples; lanes N: negative controls (no DNA template).