. 2015 Jul 31;10(7):e0134743. doi: 10.1371/journal.pone.0134743

Table 1. Primers and probes used in this study.

Oligonucleotide	Sequence (5’-3’)
16S-F (897–914)	`CGCAAGGYTRAAACTCAA`
16S-R (1066–1083)	`ATYTCACRACACGAGCTG`
16S-FAM-probe (959–977)	`FAM-ATTCGAHGCAACGCGWAGA-BHQ1`
23S-F (2438–2455)	`TACYCYGGGGATAACAGG`
23S-R (2591–2608)	`CCGAACTGTCTCACGACG`
23S-FAM-probe (2492–2509)	`FAM-TTGGCACCTCGATGTCGG-BHQ1`
IRC-CY3-probe	`CY3-CCTCACTCGAACTGGCCG-BHQ2`

F = forward; R = reverse; Y = C/T; R = A/G; H = A/T/C; W = A/T; BHQ1 = dark quencher 480–580 nm; BHQ2 = dark quencher 520–650 nm