Table 3. Internal reference control (IRC) duplex RT-PCR testing of human platelet-rich plasma spiked with E. coli.

	Number of spikedE. coli (cfu/mL)	Mean Cts of target	Mean Cti of IRC	Positive/Negative
16S	100	26.72 ± 0.21	36.77 ± 0.12	Positive: P < 0.05
	10	29.42 ± 0.27	36.61 ± 0.24	Positive: P < 0.05
	5	32.39 ± 0.43	36.17 ± 0.51	Positive: P < 0.05
	1	36.31 ± 0.64	36.39 ± 0.27	Positive*: P = 0.41
	0.5	37.26 ± 0.33	36.30 ± 0.32	Negative: P < 0.05
23S	100	25.89 ± 0.21	35.82 ± 0.25	Positive: P < 0.05
	10	29.60 ± 0.40	36.41 ± 0.15	Positive: P < 0.05
	5	31.84 ± 0.28	35.64 ± 0.37	Positive: P < 0.05
	1	35.31 ± 0.18	35.14 ± 0.17	Positive*: P = 0.12
	0.5	36.64 ± 0.44	36.01 ± 0.26	Negative: P < 0.05

*The P value showed no difference between Cts and Cti; however, the two sample pooled t-test analysis showed that Cts ≤ Cti.