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. 2015 Jul 31;10(7):e0134290. doi: 10.1371/journal.pone.0134290

Fig 4. Adiponectin regulates osteoblastogenesis in bone marrow stromal cells.

Fig 4

A) Effect of adiponectin treatment (2.5 μg/ml) from day 0–5 on osteoblast differentiation from BMSCs as assessed by Von Kossa (VK) and alkaline phosphatase staining. B) mRNA expression levels of the adiponectin receptor 1 (AdipoR1) throughout 28 days of osteoblastic differentiation. C) Effect of adiponectin treatment (2.5, 5, and 10 μg/ml) from day 14–28 on osteoblast differentiation from BMSCs as assessed by VK and alkaline phosphatase staining and on D) expression levels of osteoblast markers: osterix, Runx2, alkaline phosphatase (Al. Phosph.), osteocalcin (Osteocal.) and collagen type I (Col 1). E) Expression levels of RANKL:OPG. Colony numbers were calculated using Image J Software. All expression data were obtained by RT-qPCR analysis of RNA and GAPDH as the relative control. All data are shown as mean ± SEM from BMSCs isolated from 12 week old WT mice (n = 3–7). Statistical significance * P<0.05.