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. 2015 Jul 31;11(7):e1005079. doi: 10.1371/journal.ppat.1005079

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© 2015 Avalos-Padilla et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 8 — (A-C) SEM of pNeo (A) and pNeoEhvps32-HA (B,C) transfected trophozoites. (C) Magnification of conical helicoidally structures protruding from plasma membrane. (D-F) TEM of glutaraldehyde fixed trophozoites transfected with pNeoEhvps32-HA plasmid embedded in Polybed epoxy resin. Square in (D), magnified in (E,F). (F) A drawing in green superposed to the concentric structure in (E). (G-L) TEM of pNeoEhvps32-HA transfected trophozoites, fixed by PFA and glutaraldehyde, embedded in LR White resin and incubated with αrEhVps32 antibodies followed by gold-labeled secondary antibodies. Arrows: gold particles. e: erythrocytes fragments. (J-L) Helicoidally structures in (G-I) were drawn in green.