Figure 11.

Immunolocalization of NCKX4 in maturation stage ameloblasts. (A) ameloblasts of D11 maxillary first molars exhibited distinct subcellular localizations of NCKX4 in wild-type and null mice. While the wild-type ameloblasts showed strong NCKX4 immunoreactivity at their distal membranes, the NCKX4 signal was detected in the cytoplasm of null ameloblasts, especially in the supranuclear area. (B) In wild-type mice, maturation stage ameloblasts of D14 mandibular incisor exhibited generally strong NCKX4 signals at the distal membrane and weak in the cytoplasm. In contrast, a stronger intracellular NCKX4 immunostaining with weaker signal at distal membrane was observed in some Wdr72 null ameloblasts. The unabridged molar and incisor immunolocalizations of NCKX4 are presented in Figures S12–13. Am, ameloblasts.