Table 1.

Potential donor splice site position, splicing motif and splice scores, as determined using the Analyzer Splice Tool (http://ibis.tau.ac.il/ssat/SpliceSiteFrame.htm), within the cryptic pseudoexon 44a sequence in normal and patient DMD intron 44.

Sequence position	cDNA position	Splice site type	Motif	New potential splice site	Consensus value (0–100)
(A) Normal intron 44 region around cryptic pseudoexon 44a
30	28	Donor	GGAGTGAGG	GGAgtgagg	79.71
35	33	Donor	GAGGTGGCG	GAGgtggcg	74.79
56	54	Donor	ACTGTAACC	ACTgtaacc	69.24
76	74	Donor	CAGGTTCGA	CAGgttcga	83.84
82	80	Donor	CGAgtgatt	CGAgtgatt	71.09
108	25	Donor	gaagtacct	GAAgtacct	66.84
124	41	Donor	taggtgccc	TAGgtgccc	73.87
(B) Patient intron 44 region around cryptic pseudoexon 44a
30	28	Donor	GGAGTGAGG	GGAgtgagg	79.71
35	33	Donor	GAGGTGGCG	GAGgtggcg	74.79
56	54	Donor	ACTGTAACC	ACTgtaacc	69.24
76	74	Donor	CAGGTTCGA	CAGgttcga	83.84
82	80	Donor	CGAgtaagt	CGAgtaagt	84.57
86	3	Donor	taagtatga	TAAgtatga	76.3
113	30	Donor	ttggtactt	TTGgtactt	72.15
135	52	Donor	caggttgaa	CAGgttgaa	72.14
141	58	Donor	gaagtgaca	GAAgtgaca	72.15
164	81	Donor	taagttaaa	TAAgttaaa	67.92

DMD, dystrophin gene.