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. Author manuscript; available in PMC: 2016 Jan 30.
Published in final edited form as: Nature. 2015 Jul 6;523(7562):597–601. doi: 10.1038/nature14553

Figure 2. Secretory progenitor cells show tonic Notch2 activity at steady-state.

Figure 2

a, Schematic representation of airway epithelial cell isolation. Relative mRNA expression of Notch2 in sorted cells (n=3 mice) (middle). Percentage of each cell type per total N2ICD+ cells (right). b-e, Immunostaining for p63 (left) or FOXJ1 (right) (green), SSEA-1 (b) or SCGB1A1 (d) (cyan) and N2ICD (red). Percentage of N2ICD+ cells per total SSEA-1+ (c) or SCGB1A1+ (e) cells (n=3 mice). f, Immunostaining for eGFP (green) and N2ICD (red) in B1-eGFP mice. g, Percentage of N2ICD+ cells per total eGFP+ cells (n=3 mice). Nuclei, DAPI (blue). White arrowheads, double-positive cells. Images are representative of n=3 mice (biological replicates). * p<0.05, *** p<0.001. Error bars, means ± SEM. Scale bar, 20μm.

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