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. Author manuscript; available in PMC: 2016 Jan 30.
Published in final edited form as: Nature. 2015 Jul 22;523(7562):561–567. doi: 10.1038/nature14656

Extended Data Fig. 3. Electron density map for the overall complex and the key interfaces based on the XFEL data.

Extended Data Fig. 3

a, A 2Fo–Fc electron density map contoured at 1 σ of the arrestin finger loop, which forms the key interface with TM7 and Helix 8.

b, A 2Fo-Fc electron density map contoured at 1 σ of the loop between TM5 and TM6, which forms the key interface with the β-strand following the finger loop.

c, A 3000K simulated annealing omit map (2Fo-Fc electron density map contoured at 1 σ) calculated from the 3.8Å/3.8Å/ 3.3Å XFEL data supports the overall arrangement of the rhodopsin-arrestin complex. In all panels, the complex structure is shown with rhodopsin colored in green, arrestin in brown and T4L in yellow.

f, Stereo views of the lariat loop with a 2Fo-Fc composite omit map at 1 σ calculated from the 3.8Å/3.8Å/ 3.3Å truncated XFEL data. Key residues are labeled.