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. 2015 Aug 1;22(1):63. doi: 10.1186/s12929-015-0168-5

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© Liu et al. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Osthole suppressed inflammation and oxidation in TAA-injected rat liver. a Nuclear fractions of rat liver from different treatment groups were analyzed by Western blotting analysis. For expression of p65, JunD and Nrf-2, PCNA expression served as loading control in nuclear protein. b GSH/GSSG ratio in the livers was measured by Glutathione kit. c Levels of 4-HNE in the livers were measured using 4-HNE ELISA kit. d Levels of MDA in the livers were measured using MDA ELISA kit e qRT-PCR showed the expressions of interleukin-1β, tnf-α and inos transcripts in rats. f ELISA of LIX, CXCL1, CCL20 and CCL5 in plasma. Data are shown as mean ± SD of 6 rats in each group. *p < 0.05; **p < 0.01, compared with other groups