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. 2015 Jun 15;19(1):255. doi: 10.1186/s13054-015-0976-8

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© Zhu et al. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Nissl and TUNEL staining of the perihematomal tissue 24 h after ICH. a Coronal brain slice shows oval-shaped hematomas at the right basal ganglia. b Nissl staining showing a decrease in the number of stained cells and more severe structural damage of the neurons around the hematoma in HI animals. However, the neuronal damage in HI-HBO animals was reduced. Right arrow indicates normal neuron, up arrow indicates damaged neuron in the photos. c Quantification analysis indicating TUNEL-positive cells increased in HI animals and decreased in HI-HBO animals. The percentage of TUNEL-positive cells was expressed as the number of TUNEL-stained nuclei divided by the total number of DAPI-stained nuclei. ^* P <0.05. Bar = 100 μm