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Serum dilution optimization for both ELISA assays and FMIA. Reference serum standard was titrated 2-fold in antigen coated wells at a fixed concentration in order to gauge a maximum signal-to-noise ratio for each assay a iELISA, b bELISA, c FMIA. Arrows show the optimum dilution of swine serum from which the highest signal to noise ratio was achieved
