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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: Transplantation. 2010 Jul 27;90(2):142–149. doi: 10.1097/TP.0b013e3181e228db

Figure 2. Simvastatin maintains endothelial KLF2 and its vasoprotective target genes expression during static cold storage.

Figure 2

A) Left, KLF2 mRNA expression determined in EC exposed for 24h to vasoprotective flow followed by static preservation at 4°C for 0h, 1h, 3h and 6h in University of Wisconsin solution supplemented with simvastatin (1μM) or its vehicle. Right, representative western blot of KLF2 and α-tubulin protein expression determined in EC under indicated conditions. B) Expression of KLF2-dependent vasoprotective target genes determined in EC described above (*p<0.05 vs 0h; #p<0.01 vs. 0h. n=3). C) Expression of KLF2 and its vasoprotective target genes in EC transfected with specific siRNA against KLF2 or control, cultured for 24h under flow conditions and preserved at 4°C for 0h or 6h in University of Wisconsin solution supplemented with simvastatin (1μM) or its vehicle (#p<0.05 vs. ctrl siRNA 6h simvastatin, n=3). Insert shows western blot demonstrating KLF2 silencing efficiency.