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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: Transplantation. 2010 Jul 27;90(2):142–149. doi: 10.1097/TP.0b013e3181e228db

Figure 4. Simvastatin inhibits endothelial activation after cold storage.

Figure 4

Top, EC monolayers were cultured for 24h under vasoprotective flow, followed by the immediate addition of IL-1β 0.1 U/mL, 4h, 37°C, left), or by cold storage for 6h with simvastatin (1 μM, right) or its vehicle (center), and then treated for 4h with IL-1β (as above). All groups were then incubated with fluorescently labeled HL-60 cells. Shown are representative fields of EC monolayers (gray) and attached HL60 cells (yellow). Bottom, quantitative fluorescence analysis of bound HL-60 cells (*p<0.05 vs. no cold storage and 6h simvastatin, n=4).