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. Author manuscript; available in PMC: 2016 Jul 31.
Published in final edited form as: Circ Res. 2015 Jun 1;117(4):e41–e53. doi: 10.1161/CIRCRESAHA.117.306457

Figure 2. miR-92a suppresses PPAP2B expression by targeting its 3′UTR.

Figure 2

(A) miR-92a mimic diminished elevated PPAP2B expression induced by athero-protective waveform in human aortic endothelial cells (HAEC). (B) miR-92a mimic suppressed PPAP2B mRNA and protein levels in HAEC under static conditions. (C) miR-92a inhibitor elevated PPAP2B mRNA and protein levels in HAEC under static conditions. (D) miR-92a mimic decreased the activity of luciferase reporter containing the human PPAP2B 3′UTR in HAEC. (E) miR-92a inhibitor increased the activity of luciferase reporter containing human PPAP2B 3′UTR in HAEC. (F) Site-directed mutagenesis of miR-92a binding site in the PPAP2B 3’UTR diminished the effect of miR-92a on the luciferase reporter activity in HAEC (D,E). All data are represented as mean ± SEM. n = 3-5. * P < 0.05, t-test.