Table 2. List of primers used in real-time qRT-PCR.
S. No | Gene name | Sequence (5′- 3′) | Size (bp) | Gene acc no |
---|---|---|---|---|
1 |
Oct4 |
F: TGTACTCCTCGGTCCCTTTC |
150 |
NM_203289 |
R: TCCAGGTTTTCTTTCCCTAGC |
||||
2 |
Sox2 |
F: GCTAGTCTCCAAGCGACGAA |
144 |
NM_003106 |
R: GCAAGAAGCCTCTCCTTGAA |
||||
3 |
Nanog |
F: CAGTCTGGACACTGGCTGAA |
149 |
NM_024865 |
R: CTCGCTGATTAGGCTCCAAC |
||||
4 |
Notch 1 |
F: ATCCAGAGGCAAACGGAG |
106 |
NM_017617 |
R: CACATGGCAACATCTAACCC |
||||
5 |
Jagged 1 |
F: AAGGCTTCACGGGAACATAC |
120 |
NM_000214 |
R: AGCCGTCACTACAGATGCAC |
||||
6 |
Hes1 |
F: GAGAGGCGGCTAAGGTGTTT |
118 |
NM_005524 |
R: GTGTAGACGGGGATGACAGG |
||||
7 |
Hes3 |
F: GAAAGTCTCCCTGGCTCGTC |
146 |
NM_001024598 |
R: CCAAATAGGGAGCGCCTTCA |
||||
8 |
Hes5 |
F: AGAGAATGTGTGTGCAGAGTCC |
70 |
NM_001010926 |
R: GGTCAGACACTTGGCAGAAGA |
||||
9 |
Hey1 |
F: GACCGTGGATCACCTGAAAA |
91 |
NM_012258 |
R: TCCCAAACTCCGATAGTCCA |
||||
10 |
Abcg2 |
F: GAGCCTACAACTGGCTTAGACTCAA |
85 |
NM_004827 |
R: TGATTGTTCGTCCCTGCTTAGAC |
||||
11 |
Δnp63α |
F: AGCCAGAAGAAAGGACAGCA |
104 |
NM_001114980 |
R: CAGGTTCGTGTACTGTGGCT |
||||
12 |
Pcna |
F: GCCAGAGCTCTTCCCTTACG |
87 |
NM_002592 |
R: TAGCTGGTTTCGGCTTCAGG |
||||
13 |
Cyclin D1 |
F: TCTACACCGACAACTCCATCCG |
133 |
NM_053056 |
R: TCTGGCATTTTGGAGAGGAAGTG |
||||
14 |
Ki67 |
F: CTTTGGGTGCGACTTGACG |
199 |
NM_002417 |
R: GTCGACCCCGCTCCTTTT |
||||
15 |
Cdc20 |
F: GTTCGGGTAGCAGAACACCA |
187 |
NM_001255 |
R: CCCCTTGATGCTGGGTGAAT |
||||
16 |
Bcl2 |
F: TGGCCAGGGTCAGAGTTAAA |
143 |
NM_000633 |
R: TGGCCTCTCTTGCGGAGTA |
||||
17 |
Bax |
F: TTGCTTCAGGGTTTCATCCA |
113 |
NM_138761 |
R: AGACACTCGCTCAGCTTCTTG |
||||
18 |
Caspase 3 |
F: TGTGGCATTGAGACAGAC |
159 |
NM_004346 |
R: CATGGCACAAAGCGACTG |
||||
19 |
Caspase 9 |
F: CCAGAGATTCGCAAACCAGAGG |
88 |
NM_001229 |
R: GAGCACCGACATCACCAAATCC |
||||
20 |
Lc3a |
F: CGTCCTGGACAAGACCAAGT |
181 |
NM_181509 |
R: CTCGTCTTTCTCCTGCTCGT |
||||
21 |
Lc3b |
F: AGCAGCATCCAACCAAAA |
187 |
NM_022818 |
R: CTGTGTCCGTTCACCAACAG |
||||
22 |
Atg-7 |
F: GGATGAAGCTCCCAAGGACAT |
54 |
NM_001144912 |
R: CCAGCAGAGTCACCATTGTAGTA |
||||
23 |
Rab 7 |
F: AGTACAAAGCCACAATAGGAGC |
116 |
NM_004637 |
R: ACCGAGAGACTGGAACCGT |
||||
24 |
Lamp-1 |
F: AGTGGCCCTAAGAACATGACC |
128 |
NM_005561 |
R: AGTGTATGTCCTCTTCCAAAAGC |
||||
25 |
Lamp-2 |
F: GAAAATGCCACTTGCCTTTATGC |
173 |
NM_002294 |
R: GGTCCGAACTGCACTGCTATT |
||||
27 |
β-actin |
F: ACAGGGGAGGTGATAGCATT |
100 |
NM_001101 |
R: GACCAAAAGCCTTCATACATCTC |