. 2014 Jun 17;15(3):333–341. doi: 10.1007/s10238-014-0298-1

Table 3.

Primer sequences and PCR conditions used for real-time quantitative RT-PCR

Primer		Sequences	Length	Annealing
S100P	F	5′–TAGCACCATGACGGAACTAGAGACA–3′	182	53 °C 10 s
S100P	R	5′–TGAGCAATTTATCCACGGCATC–3′	182	53 °C 10 s
AKR1C3	F	5′–GGATTTGGCACCTATGCACCTC–3′	91	52 °C 10 s
AKR1C3	R	5′–CTATATGGCGGAACCCAGCTTCTA–3′	91	52 °C 10 s
CNN3	F	5′–TTCCATACAACCATTGACATTGGAG–3′	127	52 °C 20 s
CNN3	R	5′–GGCTGGCACATTTGTTGGTTC–3′	127	52 °C 20 s
AKR1B1	F	5′–TATTCACTGGCCGACTGGCTTTA–3′	71	60 °C 10 s
AKR1B1	R	5′–GAACCACATTGCCCGACTCA–3′	71	60 °C 10 s
MACC1	F	5′–AGGTCAGCATTGGTTTCACTAGGAG–3′	65	52 °C 20 s
MACC1	R	5′–CAATGAGACTGGAGCATGTTTGG–3′	65	52 °C 20 s
HBE1	F	5′–CTGAGTGAGCTGCACTGTGACAAG–3′	75	60 °C 10 s
HBE1	R	5′–AATCACCATCACGTTACCCAGGA–3′	75	60 °C 10 s
TFF3	F	5′–CTGCTGCTTTGACTCCAGGAT–3′	90	63 °C 10 s
TFF3	R	5′–CAGCTGGAGGTGCCTCAGAA–3′	90	63 °C 10 s
FGGY	F	5′–AGGACCTTGATGATCTTGCCATTC–3′	93	52 °C 20 s
FGGY	R	5′–CTGCTGCCTCCATGGCTTCTA–3′	93	52 °C 20 s
ACTB	F	5′–TGGCACCCAGCACAATGAA–3′	186	52 °C 10 s
ACTB	R	5′–CTAAGTCATAGTCCGCCTAGAAGCA–3′	186	52 °C 10 s