Figure 4.

Dependence of ALA synthesis on total liver protein. Livers were homogenized in 0.25 M sucrose and then diluted, 2.5 to 17.5 mg protein/mL. Samples were then analyzed for ALAS activity with the optimal concentrations of reactants (50 mM glycine, 50 μM succinylacetone, 100 μM succinyl CoA, 40 μM pyridoxal 5′-phosphate, 50 mM potassium phosphate pH 7.4).