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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: Clin Biochem. 2015 May 8;48(12):788–795. doi: 10.1016/j.clinbiochem.2015.04.023

Scheme 2.

Scheme 2

Hantzsch derivatization of δ-aminolevulinic acid. δ-Aminolevulinic acid reacts with 2 molecules of acetylacetone and 1 molecule of formaldehyde to form the fluorescent derivative 2,6-diacetyl-1,5-dimethyl-7-(2-carboxyethyl)-3-pyrrolizine with an excitation maximum of 370 nm and an emission peak at 460 nm.