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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Biomaterials. 2015 Jul 14;66:83–91. doi: 10.1016/j.biomaterials.2015.07.011

Figure 4.

Figure 4

Resistance to tearing as determined by suture pullout test for Fresh, GLUT, or TRI treated leaflets subjected to no enzymatic treatment, GAGase, or elastase (6 groups, n = 8 for each group). (A) Peak stress required to cause tearing. (B – G) alcian blue staining on Fresh, GLUT, or TRI treated leaflets subjected to no enzymatic treatment or GAGase. GAGs are stained blue and nuclei are counterstained red. (H – M) Movat’s pentachrome staining on Fresh, GLUT, or TRI treated leaflets subjected to no enzymatic treatment or elastase. Collagen and elastin are stained yellow and black, respectively. *Indicates significant difference.