Table 1. Characterization Results of Synthesized Polymeric Fluorogens.
| Entry | Mn | Đ | DP | EO | KD | kON | τdc | Brightness |
|---|---|---|---|---|---|---|---|---|
|
| ||||||||
| (#) | (g/mole) | (n) | (m) | (nM) | (M-1*s-1) | (μs) | (kHz) | |
| 1 | 177,400 | 1.26 | 372 | 8.5 | 33 | 1.36E4 | 611 | 6.499 |
| 2 | 71,820 | 1.20 | 150 | 8.5 | 42 | 1.62E4 | 490 | 6.728 |
| 3 | 43,560 | 1.20 | 90 | 8.5 | 29 | 1.89E4 | 363 | 7.052 |
| 4 | 31,970 | 1.40 | 66 | 8.5 | 10 | 3.98E4 | 353 | 7.323 |
| 5 | 49,890 | 1.31 | 164 | 4.5 | 4.7 | 3.14E4 | 404 | 7.573 |
| 6 | 38,750 | 1.33 | 164 | 3.0 | 6.0 | 3.36E4 | 377 | 9.230 |
| 7 | 180,200 | 1.82 | 202 | 19.3 | -- | -- | 532 | 7.678 |
| 8 | 23,130 | 1.44 | 120 | 2.0 | -- | -- | -- | -- |
| 9a | 97,960 | 1.84 | 204 | 8.5 | 1.9 | 3.46E4 | -- | 6.855 |
| MG-Esterb | -- | -- | -- | -- | 0.15 | 8.2E6 | 217 | 7.720 |
a
MG amide initiator containing a longer linker was used instead of PBiB-MG.
b
KD and kON values were obtained from J. Mol. Biol. 2013, 425, 4595.
c
Diffusion correlation time from FCS measurements by fitting autocorrelation curves using a one component 3-D diffusion model.