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. Author manuscript; available in PMC: 2016 Aug 15.
Published in final edited form as: Exp Cell Res. 2015 May 15;336(2):276–286. doi: 10.1016/j.yexcr.2015.05.005

Figure 3. Interaction between Cavβ1a and TnT3 in C2C12 cells.

Figure 3

(A) C2C12 cells transduced with RAdCavβ1a/YFP were cultured until myotubes formed. Whole cell lysis preparations were immunoprecipitated with Cavβ1a antibody or IgG and immunoblotted. The antibody against heavy chain (HC) indicates a similar Cavβ1a antibody and IgG loading. (B) Diagram of the DsRed-conjugated TnT3 constructs. (C) Co-expression of Cavβ1a/YFP and various TnT3/DsRed constructs in C2C12 cells cultured in growth medium. Both TnFL/DsRed and TnCT/DsRed localized in the nucleus and co-localized with Cavβ1a/YFP. In contrast, Cavβ1a/YFP expression, alone or co-expressed with TnNT/DsRed, TnM/DsRed, or control DsRed, was localized throughout the cell. Scale bar = 50 μm.