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. Author manuscript; available in PMC: 2016 Aug 15.
Published in final edited form as: Exp Cell Res. 2015 May 15;336(2):276–286. doi: 10.1016/j.yexcr.2015.05.005

Figure 8. Endogenous TnT3 enriches nuclear Cavβ1a during the early differentiation of C2C12 cells.

Figure 8

Cavβ1a/YFP overexpression in undifferentiated C2C12 myoblasts was observed primarily in the cytoplasm and weakly in the nucleus (A, C, and C′). Endogenous TnT3 was detected by immunfluorescence in mononucleated, elongated cells in both cytoplasm and nucleus (B, D, and D′). In the elongated C2C12 cells at an early stage of differentiation and co-expressed with Cavβ1a/YFP, both TnT3 and Cavβ1a/YFP were observed in the nucleus; the latter was greatly enriched (D, D′) compared to the case when it only was expressed in undifferentiated myoblasts (A, C, and C′).