Table 1. Forward and reverse PCR primers used for amplification of the different TnT3 and Ca_vβ_1a domains for subcloning.

For each primer, the endonuclease restriction site used for cloning the insert into pGBKT7, pGADT7 and YFP is underlined.

pGBKT7-Cavβ1a domain primers (EcoRI-BamHI)
Cavβ1a Forward-1	5′-GCTAGAATTCATGGTCCAGAAGAGCGGCATGTCC-3′
Cavβ1a Forward-58	5′-GCTAGAATTCATGGGCTCAGCAGAGTCCTACACG-3′
Cavβ1a Forward-101	5′-GCTAGAATTCATGGTGGCTTTTGCTGTTCGGACAAAT-3′
Cavβ1a Reverse-57	5′-GCTAGAATTCCTGGCGGACGAAGCTGTTGGA-3′
Cavβ1a Reverse-99	5′-GCTAGAATTCTTTGGTCTTGGCTTTCTCGAG-3′
Cavβ1a Reverse-524	5′-GCTAGAATTCCATGGCGTGCTCCTGAGGCTG-3′
pGADT7-TnT3 domain primers (NdeI-XhoI)
TnT3 Forward-1	5′-GCTACATATGATGTCTGACGAGGAAACTGAACAA-3′
TnT3 Forward-160	5′-GCTACATATGAAAAAGAAGATTCTT-3′
TnT3 Reverse-159	5′-GCTACATATG TTATTCCCGGGCTGTCTGTTT-3′
TnT3 Reverse-244	5′-GCTACATATG TTACTTCCAGCGCCCACCGACTTT-3′
Cavβ1a 100T/YFP primers (EcoRI-SalI)
Cavβ1a100T/YFP-Forward	5′-GCTAGAATTCCATGGTGGCTTTTGCTGTTCGGACAAAT-3′
Cavβ1a100T/YFP-Reverse	5′-GCTAGTCGACATGGCATGTTCCTGC-3′