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. 2015 Aug 3;6:116. doi: 10.3389/fendo.2015.00116

Figure 4.

Figure 4

Immunofluorescence analysis of white adipose tissue. Whole white adipose tissue from HF-fed WT and L-CC1 mice was stained with BODIPY 558/568 to detect lipid (gray) and with anti-F4/80 to detect macrophages (green). All images were captured using LSCM with a 20× objective and are 2D projections of a 3D image z-stack. (A) Representative picture from HF-fed WT mice. Yellow arrows show multiple areas with crown-like structures (CLS). (A’). A blow up of the area around the bottom left yellow arrow in (A) showing multiple layers of CLS containing macrophages (green) surrounding adipocytes (gray). Also visible are small pieces of lipid inside the macrophages. (B) Representative projection from HF-fed L-CC1 mice. One yellow arrow points out the single CLS detected in the image. (B’). A blow up of the single CLS in (B) (yellow arrow) surrounding one adipocyte from HF-fed L-CC1 mice. No small pieces of lipid are visible inside of macrophages. Representative images from three sections per mouse are shown.