. 2015 Jul 2;6:93–99. doi: 10.1016/j.redox.2015.06.017

Table 1.

Effect of additives on fluorimetric parameters of glycoxidative modifications of BSA. Mean+SD.

Additive	AGE	Dityrosine	N′-formylkynurenine	Kynurenine	Thioflavin T
BSA	7.19 ±4.11^*	4.99±2.74^*	7.18±4.12^*	9.26 ±4.79^*	12.06±5.47^*
BSA+ascorbic acid, no additive	100	100	100	100	100
DMSO	157.86±11.33^*	185.23±10.62^*	166.92±13.12^*	140.60±9.14^Δ	46.93±14.48^Δ

Antioxidants
Captopril 1 mM	80.04±6.15^Δ	84.22±5.78^Δ	86.37± 6.35^#	105.51±7.62	77.06±11.53^#
Tiron 1 mM	47.00±7.15^*	48.45±10.40^Δ	40.03±5.67^*	100.56±3.23	136.80±24.19
Trolox 1 mM	46.23 ±0.38^*	35.35±0.52^*	44.07±1.48^*	110.14±1.41^*	35.59±26.85^#

Metal chelators
EDTA 1 mM	126.99±6.04^Δ	135.29±7.76^Δ	130.02±7.38^Δ	134.79±7.81^Δ	186.99±15.27^*
EGTA 1 mM	101.81±2.65	102.72±5.81	105.25±8.94	95.68±6.53	73.25±6.04^Δ
DTPA 1 mM	145.12±8.08^*	156.67±5.78^*	149.57±6.02^*	148.85±16.85^Δ	31.93±39.78#
NTA 1mM	110.41±12.98	119.76±12.84	113.27±10.90	111.70±11.36	176.30±31.27^#

Organic acids
1-Cyano-4-hydroxycinnamic acid 1 mM	4.24±0.20^*	3.07±0.16^*	3.32±0.47^*	9.71±0.81^*	64.01±20.36^#
4-Hydroxycinnamic acid 1 mM	107.35±6.65	109.01±6.04	111.26±7.49	110.73±7.30	55.30±18.55^#

Polyphenols
Caffeic acid 1 mM	<0^*	<0^*	<0^*	<0^*	261.65 ± 20.65^*
Ellagic acid 1 mM	52.71±0.81^*	28.62±0.45^*	46.94±0.62^*	89.63±2.07^*	519.37±29.54^*
Ferulic acid 1 mM	<0^*	15.43±4.28	<0^*	166.44±3.25	45.16±12.69^Δ
Gallic acid 1 mM	191.78 ±3.17^*	105.68±3.46^#	188.74 ±4.13^*	284.02±8.56	176.77 ±16.98^Δ
Genistein 1 mM	8.74 ±0.42^*	6.43± 0.35^*	7.85 ± 0.45^*	16.90±0.40^*	359.27±25.08^*
Genkwanin 1 mM	25.89±5.12^*	18.95 ±3.25^*	25.41 ±5.48^*	23.91 ±9.77^*	<0^*
Naringin 1 mM	20.18 ± 0.72^*	13.12 ± 0.35^*	17.47 ± 0.43^*	99.14 ± 1.28	319.05 ± 25.32^*
Propyl gallate 1 mM	<0^*	<0^*	<0^*	70.17 ±2.08^*	233.83±32.31^Δ
Quercetin 1 mM	21.21±0.10^*	11.21±0.14^*	19.82±0.10^*	79.60±1.90^*	403.74 ±28.77^*
Rutin 1 mM	2.61 ±0.22^*	1.48±0.13^*	1.76±0.25^*	3.08±0.16^*	7.56 ±3.80^*

Oxidants
AAPH 5 mM	367.67±10.24^*	349.04±9.40^*	374.63±12.31^*	525.06±18.21^*	689.92±13.59^*
FeCl₃ 1 mM	57.77±1.54^*	64.12±1.33^*	64.00±2.87^*	94.02±1.66^Δ	180.82±12.86^*
H₂O₂ 0.1 mM	101.86 ±7.17	99.78±7.24	98.15±6.87	100.19±5.19	124.15±14.87^#

Nitroxides
TEMPO 0.05 mM	116.18±3.51^Δ	120.31±4.68^Δ	118.30±3.77^Δ	104.61±4.75	134.19±14.23^Δ
TEMPO 0.1 mM	111.52±5.12^#	114.20 ±3.14^Δ	110.01±5.24^#	101.19 ±4.68	82.81±20.48
TEMPO 0.2 mM	110.97 ± 4.49^#	113.17 ± 3.93^Δ	111.73 ± 2.01^*	101.61 ± 3.58	144.07 ± 18.13^Δ
TEMPO 0.5 mM	102.98±5.56	104.50±4.26	105.19 ±4.85	91.31 ±5.18#	139.83±15.44^Δ
TEMPO 1 mM	97.27 ±4.54	98.39±4.17	100.15 ± 5.22	85.87±5.6^#	138.61 ±19.08^Δ
4-Hydroxy-TEMPO 0.05 mM	128.54 ± 6.06^Δ	128.18 ± 3.52^*	127.75 ± 4.21^*	118.00 ± 6.64^Δ	114.30 ± 11.47
4-Hydroxy-TEMPO 0.1 m mM	122.91 ±6.78^Δ	123.93±5.67^Δ	123.68 ± 4.63^*	105.89 ±5.65	75.75 ±15.24
4-Hydroxy-TEMPO 0.2 mM	119.29 ± 5.46^Δ	123.14 ± 4.11^*	120.58 ± 4.68^Δ	108.89 ± 5.24^#	124.05 ±14.73^Δ
4-Hydroxy-TEMPO 0.5 mM	117.19 ± 7.77^#	118.36 ±5.43^Δ	117.86 ±6.76^#	102.44 ±4.19	176.33 ± 13.67^*
4-Hydroxy-TEMPO 1 mM	113.32 ±12.39	112.37 ±9.83	110.50±6.87	100.24±9.18	145.35±19.59^Δ
4-Carboxy-TEMPO 0.05 mM	93.79 ±4.53	90.36 ±2.67^Δ	91.83 ±2.7	100.19±3.44	380.08 ±30.19^*
4-Carboxy-TEMPO 0.1 m mM	108.40±3.61^#	104.56±1.99^#	106.09 ±2.4^#	114.03±1.8^*	328.22 ±42.91^*
4-Carboxy-TEMPO 0.2 mM	120.19±5.37^Δ	118.63±5.74^Δ	119.83 ±3.88^*	119.74±5.15^Δ	336.72 ±38.29^*
4-Carboxy-TEMPO 0.5 mM	120.76±2.18^*	120.06±2.18^*	119.80±3.28^*	115.26±3.93^Δ	133.4 ±23.03
4-Carboxy-TEMPO 1 mM	120.26±2.69^*	119.39 ±2.07^*	118.43 ±2.38^*	111.50±1.74^*	122.41±21.2

Compounds written in italics were dissolved in DMSO and compared with samples incubated with AA and DMSO.

p<0.001 (with respect to BSA incubated with AA alone).

^Δ

p<0.01 (with respect to BSA incubated with AA alone).

p<0.05 (with respect to BSA incubated with AA alone).