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Endogenous hsp104 was deleted and substituted with plasmid-expressed Hsp104 under control of a GPD (high), ADH1 (medium), or SUP35 (low) promoter. Cells expressing mCherry-tagged luciferase from a plasmid and GFP-tagged Edc3 from the endogenous locus were exposed to a robust heat shock at 46°C and then transferred back to 25°C. Related to Figure 7.
