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. 2015 Aug 4;4:e06807. doi: 10.7554/eLife.06807

Video 13. Edc3 co-aggregates with luciferase in a Hsp104-dependent manner.

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DOI: 10.7554/eLife.06807.052

Endogenous hsp104 was deleted and substituted with plasmid-expressed Hsp104 under control of a GPD (high), ADH1 (medium), or SUP35 (low) promoter. Cells expressing mCherry-tagged luciferase from a plasmid and GFP-tagged Edc3 from the endogenous locus were exposed to a robust heat shock at 46°C and then transferred back to 25°C. Related to Figure 7.

DOI: http://dx.doi.org/10.7554/eLife.06807.052