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. 2015 Jul 16;113(3):433–442. doi: 10.1038/bjc.2015.229

Figure 1.

Figure 1

Overexpression of SOCS1 inhibits GC cell growth. (A) Effect of AdSOCS1 on viability of five GC cell lines. Cells were infected with either AdSOCS1 or AdLacZ (0–160 multiplicity of infection (MOI)). Growth ratios for AdLacZ- and AdSOCS1-infected cells were calculated as the percentage of absorbance readings for infected cells relative to that of untreated cells. Statistical analyses were performed using Welch's t-test and two-sided P values less than 0.05 were considered significant. Values shown represent the average±standard deviation (s.d.) of hexaplicate wells. (B) Effect of AdSOCS1 on apoptosis. Activation of apoptosis was evaluated by caspase-3 fluorometric assay. Cells were infected with either AdSOCS1 or AdLacZ (0–160 MOI). After 48 h, enzymatic activity of caspase-3 was measured. Statistical analyses were performed using Welch's t-test and two-sided P values less than 0.05 were considered significant. Values shown represent average±s.d. of triplicate wells. (C) Expression of SOCS1, phospho-STAT3, and cleaved caspase-3 after transduction of AdSOCS1.