Table 2. Pyrosequencing serum percent GADD45a methylation in four CpGs by biopsy result.
|
CpG P1 |
CpG P2 |
CpG P3 |
CpG P4 |
Sum of four CPGs |
||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Cancer | Benign | Cancer | Benign | Cancer | Benign | Cancer | Benign | Cancer | Benign | |
| N | 34 | 48 | 34 | 48 | 34 | 48 | 34 | 48 | 34 | 48 |
|
Percent GADD45a methylation | ||||||||||
| Min | 1.10 | 0.00 | 1.74 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 3.11 | 0.00 |
| Max | 73.22 | 46.85 | 92.00 | 57.60 | 50.30 | 52.68 | 52.52 | 46.79 | 235.08 | 203.92 |
| s.d. | 21.16 | 8.91 | 23.27 | 10.73 | 14.83 | 8.51 | 13.86 | 7.31 | 70.72 | 32.69 |
| Median | 11.60 | 4.02 | 8.99 | 4.81 | 7.06 | 1.93 | 5.75 | 2.16 | 31.59 | 14.91 |
| Median difference | 7.58
P=0.005 |
4.18
P=0.0031 |
5.15
P=0.001 |
3.59
P=0.0017 |
16.68
P=0.002 |
|||||
| Mean | 21.42 | 7.19 | 22.20 | 8.44 | 14.37 | 4.63 | 12.23 | 4.00 | 70.22 | 24.25 |
| Mean difference | 14.23
P=0.006 |
13.76
P=0.0025 |
9.75
P=0.0012 |
8.23
P=0.0027 |
45.97
P=0.001 |
|||||
|
Log base 2-transformed data | ||||||||||
| Min | 0.14 | −1.00 | 0.80 | −1.00 | −1.00 | −1.00 | −1.00 | −1.00 | 1.64 | −1.00 |
| Max | 6.19 | 5.55 | 6.52 | 5.85 | 5.65 | 5.72 | 5.71 | 5.55 | 7.88 | 7.67 |
| s.d. | 1.75 | 1.56 | 1.74 | 1.55 | 2.19 | 1.85 | 2.15 | 1.77 | 1.84 | 1.63 |
| Median | 3.54 | 2.00 | 3.17 | 2.26 | 2.74 | 0.93 | 2.52 | 1.11 | 4.96 | 3.90 |
| Mean | 3.54 | 2.05 | 3.56 | 2.28 | 2.68 | 1.04 | 2.43 | 0.93 | 5.19 | 3.77 |
| Mean difference | 1.50 P=0.0001 | 1.28 P=0.0008 | 1.64 P=0.0005 | 1.50 P=0.0009 | 1.42 P=0.0004 | |||||
P-value from two-sample Student's t-test.