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. 2015 Jul 9;113(3):414–424. doi: 10.1038/bjc.2015.254

Figure 2.

Figure 2

IMP3 and Lin28B knockdown resulted in reduction of proliferation, migration, and invasion in ovarian cancer BG-1 cells. (A, B) Cell proliferation of shControl cells (shControl), Lin28B-knockdown cells (shLin28B-860, shLin28B-508) and shIMP3-knockdown cells (shIMP3-268, shIMP3-596). Knockdown of Lin28B or IMP3 was confirmed by western blot analysis (inserts). Each point on the curve represents the mean cell number±s.e.m. (n=3). *P<0.05, compared with shControl. Comparisons of the migration (C) and invasion (D) activities between the shControl BG-1 cells (shControl), Lin28B-knockdown BG-1 cells (shLin28B-860, shLin28B-508) and shIMP3-knockdown BG-1 cells (shIMP3-268, shIMP3-596) determined by in vitro Transwell invasion and migration assays. Relative migration and invasion activities in Lin28B- or IMP3-knockdown cells were calculated in relation to the number of cells in the shControl group. Each group was plated in triplicate in each experiment, and each experiment was repeated at least three times. *P<0.05, compared with controls. Bar=100 μm.