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. 2015 Aug 3;5:12645. doi: 10.1038/srep12645

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Copyright © 2015, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(A) Fluorescence (upper) and bright field (lower) images of wild type (WT) and transgenic (TG) kidney, liver, heart atria, ventricles and lung. (B) Immunohistochemistry of transgenic brain, spleen, lung, renal cortex, liver, and heart ventricle at lower magnification (scale bars represent 100 μm). (C) Immunohistochemistry of transgenic liver, renal cortex, heart ventricle and atrium at higher magnification (scale bars represent 50 μm). Immunostaining of the GCaMP2 was performed by a GFP-recognizing antibody. Hoechst (Hst) was used to stain the nuclei.