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. 2015 Aug 3;5:12645. doi: 10.1038/srep12645

Figure 4. CAG-GCaMP2 expressing cardiomyocyte cultures allow detection of ligand-induced calcium signals and drug-induced alteration of physiologically relevant ligand-induced calcium signals.

Figure 4

(A) The effects of adrenalin, ATP, ionomycin and EGTA administration are shown on the kinetics of calcium signals in atrial (left panel) and in ventricular (right panel) cardiomyocyte cultures. (B) Ligand-induced calcium signals in wild type (WT) primary cardiac cultures loaded with the calcium-sensitive Fluo-4 AM dye. (C) The effects of 6.0 μM terfenadine (left panel) or 10.0 μM terodiline (right panel) followed by adrenalin, ATP, ionomycin and EGTA administration are shown on the kinetics of calcium signals in ventricular cell cultures. (D) The effects of 37.5 μM mefloquine, as well as adrenalin, ATP, ionomycin and EGTA administration are shown on the kinetics of calcium signals in atrial (left panel) and in ventricular (right panel) cell cultures. For each measurement, a total number of at least 25 cells was examined; light gray lines represent S.E.M. values.