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. 2015 Aug 3;5:12748. doi: 10.1038/srep12748

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Copyright © 2015, Macmillan Publishers Limited

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(a) Bovine PBMC isolated from non-BNP (n = 12) and BNP (n = 10) dams were stained with IgG isolated from the colostrum or serum of two dams. PBMC and IgG donors were MHC class I haplotyped and haplotyping results are listed online in Supplementary Table SI. (b) Cells from different species were stained with IgG isolated from BNP dams. Data are representative for three different BNP Ab donors. COS-7 = African green monkey. CHO-K1 = Chinese hamster. SP-20 = Mouse. THP-1 = Human. HEK-293 = Human. Live cells were gated on forward and sideward scatter. (c) PBMC isolated from three dams were cross-stained with IgG isolated from the same animals to test whether alloantibodies recognize autoantigens. The horizontal dotted line depicts the average GMFI of PBMC staining by autologous IgG. In all experiments Ab staining was measured using flow cytometry. GMFI = Geometric Mean Fluorescent Intensity.