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. 2015 Aug 3;5:12748. doi: 10.1038/srep12748

Figure 2. BNP alloantibodies recognize MHC class I alleles carried by the cell line used for vaccine production.

Figure 2

(a) 29 full length MHC class I clones derived from MDBK cells, the cell line used for the production of Pregsure© BVD, were sequenced and the relative number of clones per identified allele are depicted. (b,c) Full length MDBK-derived MHC class I clones or a mock construct were transfected into HEK293 cells, stained with anti-bovine MHC class I mAb ILA88 as a positive control and IgG isolated from the serum of one (b) or four (c) BNP dam(s) and Ab binding was revealed using flow cytometry. BNP 1 in c is the same dam as in b. Results are based on two separate transfection experiments. aDenotes a local name (Genbank accession number KM397368).