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. 2015 Aug 3;5:12759. doi: 10.1038/srep12759

Figure 2. Impact of Akt1 and Akt2 silencing on the proliferation of LNM35 cells.

Figure 2

LNM35 cells were transiently transfected with siRNAs targeting the transcripts encoding Akt1, Akt2 or with its siControl sequences. On days 2, 3 and 5 following the transfection, cancer cell proliferation was quantified in silenced Akt1 (A) and Akt2 (B) cells and compared to the growth curve of the control siRNA cells. LNM35 cells stably transduced by control-shRNA, AKT1-shRNA (Akt1-shRNA1 and Akt1-shRNA2) (C) or control-shRNA, AKT2-shRNA (Akt2-shRNA1 and Akt2-shRNA2) (D) were seeded into six-well tissue culture dishes (50,000 cells /dish) and counted daily for 5 days. Data are means+/−SEM from 3 independent experiments. Statistical differences obtained at *P < 0.05, **P < 0.01 and ***P < 0.001.