Figure 3. Impact of Akt1 and Akt2 silencing on LNM35 colonies’ growth.

Anchorage-independent colonies growth of LNM35 cells stably silenced for Akt1 (A) and Akt2 (C) is shown. Cells (5×10³) were plated in 0.3% soft agar. Three weeks later, colonies were stained with Giemsa and scored. Data are from three experiments performed in duplicate. The colonies formed in soft agar from the two respective silencing designs were photographed after three weeks (B and D). Data are means +/−SEM from 3 independent experiments. Statistical differences obtained at ***P < 0.001.