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. 2015 Jul 30;6:7858. doi: 10.1038/ncomms8858

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(a) VWF, when labelled with antibodies, resisted being cleaved by ADAMTS13 and trapped blood cells including platelets and leukocytes. (b,c) Zoomed images of microvessels in the dashed box in a, showing VWF fibres with bound platelet and leukocytes in the vessel lumen after 30 min of blood perfusion. (i) Bright-field images and (ii,iii) immunofluorescence projected z-stack images. In particular, (bii), (biii) and (cii) were z-stack projected images of planes from the centre plane to the wall, and (ciii) was z-stack projected image of centre planes of 20 μm thickness. Red: CD31, green: VWF, magenta: CD41a and blue: nuclei. N >3.