Table 1.

PCR amplification and sequencing of PknB and PK genes of S. aureus ATCC 12600

Gene	Primers	Conditions	Product size	Gene accession numbers
PknB	FP: 5′-CATGATAGGTAAAATA-3′ RP: 5′-TTATACATCATCATA-3′	Initial denaturation: 94 °C for 10 min. 40 cycles of denaturation: 94 °C for 60 s annealing: 29.2 °C for 30 s. amplification: 72 °C for 120 s and final elongation: 72 °C for 10 min	2 kb	JN695616
PK	FP: 5′-CGACCAGCTTCAGAATC-3′ RP: 5′-GAGCAGCATCAATCGT-3′	Initial denaturation for 10 min at 94 °C; 40 cycles of each having denaturation at 94 °C for 60 s, annealing at 43 °C for 35 s and amplification at 72 °C for 120 s final extension step at 72 °C for 5 min	1.6 kb	JN645815