Table 1.

RT-PCR conditions for amplification of DXS and crtB genes

Step	Time	Temp (°C)
Reverse transcription	30 min	55
Initial PCR activation	5 min	95
35 cycles
Denaturation	30 s	95
Annealing–(DXS/crtB)	30 s	51
Extension	45 s	72
Final extension	10 min	72
Final hold	∞	4