Fig. 1.

eIF4E/m⁷GTP/4E-BP1 complex. (A) Sequence of 4E-BP1. Relevant motifs (underlined), phosphorylation sites (stars), and constructs used in this study (lines of different length) are indicated. (B) 4E-BP1 flexibility probed by relaxation studies. R₁ (Left), R₂ (Middle)_, and heteronuclear NOE (Right) data are shown for a sample of 400 μM ¹³C,¹⁵N-4E-BP1_44–87 bound to eIF4E/m⁷GTP in 95:5 H₂O/D₂O at pH 6.5. Data points corresponding to unassigned residues or residues that could not be measured are shown in black and given an arbitrary negative value. (C) Ribbon diagram (side view) of eIF4E_33–217/m⁷GTP/4E-BP1_50–84. eIF4E and 4E-BP1 are shown in blue and red, respectively. (D) Ribbon diagram of the complex described in C, but showing a dorsal view of the complex.