Fig. 3.

Effects of 4E-BP1’s C-terminal loop. (A) Cells were transfected with plasmids encoding GFP or the 4E-BP1 fragments described in Fig. 1A (FL, full-length), and eluates of an m⁷GTP column were analyzed by Western blotting. (B) Diagram representing a dual-luciferase reporter system where an IRES (EMCV) is inserted between genes encoding the Firefly luciferase (Fluc) and the Renilla luciferase (Rluc). Cells were cotransfected with this plasmid and with the GFP/4E-BP1 constructs described in A. (C) Cells were transfected with the GFP/4E-BP1 constructs described in A. GFP-positive cells were gated and analyzed for DNA content. The percentage of G1, G2, and S phase cells are shown.