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15N HSQC of eIF4E/m7GTP and 4E-BP144–87 in the complex form. 2D [1H, 15N] TROSY-HSQC spectrum of eIF4E (A) and 4E-BP144–87 (B) in the bound form acquired on a 900-MHz spectrometer (∼0.4 mM in 25 mM sodium phosphate, 50 mM NaCl, 2 mM DTT buffer at pH 6.5).
