Fig. 2.

Rnaseh1 ablation leads to the accumulation of free 5′ ends of DNA mapping to nucleotide position ∼15,600 of the heavy strand of mouse mtDNA. EciI digested and denatured [80% (vol/vol) formamide, 15 min at 85 °C] MEF mtDNAs were fractionated by 1D-AGE and blot hybridized to riboprobe H15007-15805. Where indicated, samples were treated with (+) or without (−) Eco-RNase HI before denaturation. ΔRH1, mtDNA from MEFs treated with 100 nM 4HT for 8 d; Con, control MEFs. Interpretations of the single-stranded products are illustrated beside the gel images; red lines, RNA; black lines, DNA. Markers are shown in SI Appendix, Fig. S3.