Fig. 3.

Persistent RNA patches after Rnaseh1 ablation revealed by a combined RNase H and single-stranded nuclease treatments. (A) MEF mtDNA from cells culture for 7 d without (control) or with (ΔRH1) 4HT was digested with DraI, and where indicated additionally with single stranded nuclease (SSN) and Eco-RNase HI (RH), before native 1D-AGE, 1% agarose TAE. Fragments were blot hybridized to riboprobes H14881-15490 and H15450-16030. Interpretations of the products are illustrated beside the gel images; red lines, RNA; black lines, DNA. A truncated fragment mapping to nt ∼15,200 (*) was not reproducible (SI Appendix, Fig. S5I).