Fig. 5.

Loss of Rnaseh1 leads to retention of a RNA patch on the L-strand close to LSP. MEF mtDNA from cells cultured for 10 (A) or 8 (B) d with 4HT (ΔRH1) or from MEFs cultured without drug was digested with BamHI and PstI (A) or HincII (B), denatured for 15 min at 85 °C, in 80% (vol/vol) formamide, fractionated by 1D-AGE for 6 h at 11 V/cm, and blot hybridized to the indicated L-strand probe. RH, samples were treated with (+) or without (−) Eco-RNase HI. (A) 1% agarose Tris-acetate gel. (B) 2.3% (wt/vol) agarose sodium borate gel. Markers are based on PCR products of defined lengths (SI Appendix, Fig. S6).